Abstract：The promoter fragment of the F3'5'H gene of Lycium ruthenicum Murr. and its albino
Fruit was cloned by 3times of genome walking by Genome Walking Kit (TaKaRa) and
sequenced. The bioinformatics analysis showed that the sequence homology of two promoters
was 90.3%, the sequences were submitted to PlantCare to predict the promoter elements.
The results showed that both promoters have TATA-Box, CAAT-Box, TC-rich repeats,
WUN-motif, Sp1, Box I, G-box, skin-1 and ARE. But promoter TGACG-motif predicted in
Lycium ruthenicum Murr. was not predicted in the albino fruit promoter.
With 2promoters combined with the GUS reporter gene to construct the plant transient
expression vector, and transient transformed tobacco by Agrobacterium -mediated Method,
histochemical staining was performed to determine the promoter activity. The results showed that both promoters had activities. Then the GUS gene expression level was analyzed by Realtime
PCR. The results showed that the GUS gene expression level driven by Lycium ruthenicum
Murr. promoter was 3.09times of its albino fruits.