Abstract：In order to investigate the antiviral activity of the fusion protein of Canine interferon
and thymosin, the gene fragments of Canine interferon-α1 (IFNα1) and thymosin α1 (THYα1)
were synthesized and ligated by a linked peptide based on the known gene sequences of Canine
interferon-α1 (IFNα1) and thymosin α1 (THYα1). The gene was cloned into pPIC9Kvector, and
the recombinant plasmid pPIC9K-IFNα1-THYα1 was constructed. The recombinant plasmid
was transferred into Pichia Pastoris receptive cell GS115and cultured at 30 ° C for 18hours.
The cultured plasmid was induced by methanol for 5 days at the OD600 nm value of 4 to make the
final concentration to 1%. Based on SDS-PAGE and Western-blot results, the recombinant
protein of Canine IFNα1-THYα1 with high-purity was obtained successfully. In vitro activity
detection of target protein by MDCK / VSV virus detection system indicates that the protein
has antiviral activity.
